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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1982-8-7
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pubmed:abstractText |
Alkaline phosphatase activity was assayed by a microtiter assay (with p-nitrophenylphosphate used as substrate) in the plasma membrane of mouse spleen cells activated in vitro by the B cell mitogen LPS and the T cell-dependent B cell mitogen, PWM. No activity was detected in spleen cells cultured in the presence of the T cell mitogens PHA and Con A. This alkaline phosphatase activity was detected in the blast-enriched 30 to 40% fraction of discontinuous Percoll gradients in LPS-treated cultures, and the enzymatic activity assayed was susceptible to inhibition by the alkaline phosphatase inhibitors EDTA and L-phenylalanine. These data support the idea that the membrane alkaline phosphatase activity could be used as a marker for activated B cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6806366-Alkaline Phosphatase,
pubmed-meshheading:6806366-Animals,
pubmed-meshheading:6806366-B-Lymphocytes,
pubmed-meshheading:6806366-Cell Line,
pubmed-meshheading:6806366-Cell Membrane,
pubmed-meshheading:6806366-Edetic Acid,
pubmed-meshheading:6806366-Hydrogen-Ion Concentration,
pubmed-meshheading:6806366-Lymphocyte Activation,
pubmed-meshheading:6806366-Lymphoma,
pubmed-meshheading:6806366-Mice,
pubmed-meshheading:6806366-Mice, Inbred AKR,
pubmed-meshheading:6806366-Phenylalanine,
pubmed-meshheading:6806366-Plasmacytoma
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pubmed:year |
1982
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pubmed:articleTitle |
Alkaline phosphatase activity as a membrane marker for activated B cells.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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