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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0020792,
umls-concept:C0021764,
umls-concept:C0023418,
umls-concept:C0024264,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0205460,
umls-concept:C0205474,
umls-concept:C0220905,
umls-concept:C0567416,
umls-concept:C1880022
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pubmed:issue |
6
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pubmed:dateCreated |
1981-3-24
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pubmed:abstractText |
To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T cell growth factor), 16 human T and B leukemia cell lines were screened for constitutive and mitogen-stimulated IL-2 production. We found that the T cell leukemia line designated Jurkat-FHCRC produced > 200 U/ml of IL-2 activity after a 24-h stimulation with T cell mitogens. Peak mitogen-induced IL-2 activity was found in supernates harvested from 24-h Jurkat-FHCRC cell cultures stimulated with either 1% phytohemagglutinin or 20 microgram/ml concanavalin A. Addition of the fatty acid derivative phorbol myristate acetate to mitogen-stimulated cultures increased Jurkat-FHCRC IL-2 production to concentrations > 400 U/ml. IL-2 activity observed in such cases represented between 100--300 times that produced in conventional cultures of mitogen- or alloantigen-stimulated normal human peripheral blood or splenic lymphocytes. Jurkat-FHCRC-derived conditioned medium demonstrated equal capacity to promote the sustained in vitro proliferation of either murine or human activated T cell lines confirming the ability of Jurkat-FHCRC cells to produce human IL-2. These studies identify a new source of human IL-2 and establish a valuable reagent for the isolation and further molecular characterization of this immunoregulatory molecule.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-145543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-149174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-307029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-308962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-308989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-315987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-316981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-6444967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-6770028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-6965520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-7351241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-7359085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6778951-92524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1709-19
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6778951-Animals,
pubmed-meshheading:6778951-B-Lymphocytes,
pubmed-meshheading:6778951-Cell Line,
pubmed-meshheading:6778951-Dose-Response Relationship, Drug,
pubmed-meshheading:6778951-Humans,
pubmed-meshheading:6778951-Interleukin-1,
pubmed-meshheading:6778951-Leukemia, Lymphoid,
pubmed-meshheading:6778951-Mice,
pubmed-meshheading:6778951-Mitogens,
pubmed-meshheading:6778951-Protein Biosynthesis,
pubmed-meshheading:6778951-Rats,
pubmed-meshheading:6778951-T-Lymphocytes,
pubmed-meshheading:6778951-Tetradecanoylphorbol Acetate
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pubmed:year |
1980
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pubmed:articleTitle |
Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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