Linked Life Data

6775084

Source:http://linkedlifedata.com/resource/pubmed/id/6775084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-12-16
pubmed:abstractText
4-tert-Butylcatechol (TBC) is an antioxidant widely used in industry and a potent depigmenting agent to the skin of the workers. In this study, tyrosinase was extracted from tissue-cultured human melanoma cells and purified by polyacrylamide gel electrophoresis. T1 and T2 tyrosinase, which migrated differently on the gels, were treated with TBC as well as other depigmenting agents and natural substrates of tyrosinase. Changes in the enzyme activity on dopa oxidation were quantified by photometric and radiometric analysis. The enzyme activity was inhibited by TBC and hydroquinone, but only at a high concentration that is toxic to tissue-cultured melanocytes. The activity was also decreased by tyrosine, but was increased by dopa, particularly at a lower concentration. The results indicated that the reported methodology is useful for testing the effects of chemicals with depigmenting or pigmenting potential. TBC and hydroquinone are inhibitors of tyrosinase at concentrations higher than 1 x 10(-3) M. Dopa and tyrosine alter tyrosinase activity in the second step of melanogenesis in the same manner that has been reported to occur in the first step-conversion of tyrosine to dopa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0098-4108
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
559-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Inhibition of tyrosinase activity by 4-tert-butylcatechol and other depigmenting agents.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.