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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1980-10-24
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pubmed:abstractText |
Chymosin (Rennin) was effectively purified using an AH-Sepharose 4B column. Binding of Streptomyces pepsin inhibitor (acetul-pepstatin) with chymosin was studied spectroscopically. The binding caused ultraviolet difference and CD spectral changes suggesting microenvironmental changes around tryptophan and/or tyrosine residue(s) in chymosin. The fluorescence intensity of a hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate, increased in the presence of chymosin and was further amplified when Streptomyces pepsin inhibitor was added to the chymosin-2-p-toluidinylnaphthalene-6-sulfonate solution. The binding and dissociation-rate constants between chymosin and the inhibitor were determined using 2-p-toluidinylhnaphthalene-6-sulfonate as a probe. The binding constant was determined from the binding and dissociation-rate constants, to be 3.1 . 10(7) M-1 at 25 degrees C, pH 5.5.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-toluidino)-6-naphthalenesulfoni...,
http://linkedlifedata.com/resource/pubmed/chemical/Chymosin,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenesulfonates,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pepsin A,
http://linkedlifedata.com/resource/pubmed/chemical/Pepstatins,
http://linkedlifedata.com/resource/pubmed/chemical/Streptomyces pepsin inhibitor
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
614
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
144-50
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:6772226-Chymosin,
pubmed-meshheading:6772226-Kinetics,
pubmed-meshheading:6772226-Naphthalenesulfonates,
pubmed-meshheading:6772226-Oligopeptides,
pubmed-meshheading:6772226-Pepsin A,
pubmed-meshheading:6772226-Pepstatins,
pubmed-meshheading:6772226-Streptomyces,
pubmed-meshheading:6772226-Ultraviolet Rays
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pubmed:year |
1980
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pubmed:articleTitle |
Binding of Streptomyces pepsin inhibitor (acetyl-pepstatin) with chymosin (Rennin).
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pubmed:publicationType |
Journal Article
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