Linked Life Data

6742412

Source:http://linkedlifedata.com/resource/pubmed/id/6742412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-8-7
pubmed:abstractText
Reverse-phase high-performance liquid chromatography was used for the simultaneous determination of retinol, alpha-tocopherol, lycopene, alpha-carotene, and beta-carotene in human plasma. A multiple solvent system of methanol followed by a mixture of methanol:acetonitrile:chloroform (47:42:11) provided clear separation of these compounds. With retinyl acetate as an internal standard, standard curves were developed for each compound on the basis of peak area ratios. The coefficient of variation was less than 10% in all cases within a run. Between-run reproducibility was within +/- 2 standard deviations. The method required 100 to 200 microliter of plasma and 16 min of elution time per sample. Stability studies showed plasma samples could be stored at -10 degrees C with occasional freeze-thaw cycles for 3 to 5 weeks. This method should prove useful in clinical and epidemiological work.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
340-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Simultaneous determination of plasma retinol, alpha-tocopherol, lycopene, alpha-carotene, and beta-carotene by high-performance liquid chromatography.
pubmed:publicationType
Journal Article