Linked Life Data

6736134

Source:http://linkedlifedata.com/resource/pubmed/id/6736134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-8-16
pubmed:abstractText
The effect of hydrocortisone on fibronectin synthesis was investigated in cultured skin fibroblasts. Confluent cells were treated with hydrocortisone (10(-7) M to 10(-5) M) for 2 days and labeled with [3H]proline for 24 h. Fibronectin levels in both the culture medium and the cell layer were studied by gelatin-Sepharose affinity chromatography and SDS-polyacrylamide gel electrophoresis. In control cultures of human fetal skin fibroblasts, fibronectin constituted 8% of the total labeled proteins in the medium. The proportion of fibronectin increased to 13.1% at 10(-7) M hydrocortisone, 15.5% at 10(-6) M and to 19.4% at 10(-5) M. The proportion of fibronectin associated with the cell layer remained at 2-3% of total [3H]proline-labeled proteins and did not increase with hydrocortisone exposure. The stimulating effect of hydrocortisone on medium fibronectin was also demonstrated in cultured human newborn foreskin fibroblasts and in rabbit skin fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Hydrocortisone stimulates fibronectin synthesis in cultured fibroblasts.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't