Linked Life Data

6723655

Source:http://linkedlifedata.com/resource/pubmed/id/6723655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-7-13
pubmed:abstractText
The amino acid sequences of the alpha-crystallin A chains of 28 mammalian species, representing 14 different orders, have been analyzed, mainly on the basis of amino acid compositions of the composing peptides. The alpha A sequences of chicken and a frog have been completely determined by Edman degradation. A method is described to transport eye lenses, to be used for protein sequence studies, at ambient temperature in a solution of guanidine . HCl. The number of cysteine residues in different alpha A chains could be determined by alkaline urea gel electrophoresis after aminoethylation . In some cases the alpha A chains have been isolated from total lens extracts in a single ion-exchange chromatographic step. The average rate of substitutions in the evolution of the alpha A chains is moderately slow, approximately 3 amino acid substitutions per 100 residues in 100 million years, but varies considerably in different lineages. Substitutions involving changes in charge are strongly underrepresented; the alpha A chains tend to keep their net charge constant throughout evolution. Analysis of the types of substitutions suggests a directional trend leading to an increase in functional density of alpha A in the course of evolution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
131-40
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Primary structures of the alpha-crystallin A chains of twenty-eight mammalian species, chicken and frog.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't