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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1984-7-25
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pubmed:abstractText |
The use of displacement electrophoresis (synonymous to isotachophoresis, steady-state stacking, and moving boundary electrophoresis) for recovery of DNA fragments from agarose and polyacrylamide gels is described. Complete recovery of DNA molecules ranging from oligonucleotides to 20 000-basepairs-long fragments was achieved. The DNA is recovered in a small volume (0.1-0.3 ml) and can be used directly in enzyme-mediated cleavage and ligation reactions. The recovered DNA contained no inhibitory contaminants as revealed by ligation or restriction enzyme cleavage.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0006-3002
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
16
|
pubmed:volume |
782
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
120-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6722161-Base Composition,
pubmed-meshheading:6722161-Base Sequence,
pubmed-meshheading:6722161-DNA,
pubmed-meshheading:6722161-DNA, Recombinant,
pubmed-meshheading:6722161-Electrophoresis, Agar Gel,
pubmed-meshheading:6722161-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6722161-Oligodeoxyribonucleotides,
pubmed-meshheading:6722161-Oligonucleotides,
pubmed-meshheading:6722161-Plasmids
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pubmed:year |
1984
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pubmed:articleTitle |
Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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