Linked Life Data

6712948

Source:http://linkedlifedata.com/resource/pubmed/id/6712948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-6-7
pubmed:abstractText
A number of fluorescent peptide-lipid conjugates have been synthesized. Peptides with ten or eleven amino acids are linked through a single lysine residue to the headgroup of phosphatidylethanolamine, fluorescently labelled on one acyl chain, using homobifunctional disuccinimidyl crosslinking reagents. Peptide-lipids can be further derivatized with the hapten dinitrophenyl. Purified peptide-lipids have been incorporated into dimyristoylphosphatidylcholine monolayers at the interface of air and phosphate-buffered saline, at concentrations of up to 11 mol%. For equal average molecular areas, monolayers containing peptide-lipids have higher surface pressures than pure lipid monolayers; for equal surface pressures, peptide-lipid monolayers have higher average molecular areas than pure lipid monolayers. When the peptide-lipid monolayers are transferred to hydrophobic glass slides, the fluorescence appears uniformly distributed. Fluorescence recovery after photobleaching measurements indicate that peptide-lipids diffuse in the monolayer with coefficient 1.5 X 10(-9) cm2/s, which is much smaller than that of typical lipids in fluid membranes. In addition, the diffusion coefficient of peptide-lipids decreases with increasing peptide-lipid concentration. We conclude that the peptide portion of the peptide-lipid associates with the lipid monolayer and/or that peptide-lipids oligomerize.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
772
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Covalent linkage of a synthetic peptide to a fluorescent phospholipid and its incorporation into supported phospholipid monolayers.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't