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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1984-5-14
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pubmed:abstractText |
Monoclonal antibodies have been produced using two haptens, zeranol-7-hemisuccinate coupled to bovine serum albumin and zeranol-16-carboxypropyl ether coupled to human serum albumin. An assessment of cross-reactivity demonstrated that the monoclonal antibody raised against the 7-hemisuccinate derivative reacted with zeranol (100%), talernol (12%), zearalenone (17%), zearalanone (100%) and alpha- and beta-zearalenol (17% and less than 0.01%, respectively). In contrast the antibodies to the 16-carboxypropyl ether derivative reacted only with zeranol (100%) and also with a alpha-zearalenol (13-16%). All monoclonal antibodies were more specific than the polyclonal antibodies raised to the same haptens by conventional methods using sheep.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0140-7783
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6708163-Animals,
pubmed-meshheading:6708163-Antibodies, Monoclonal,
pubmed-meshheading:6708163-Cell Fusion,
pubmed-meshheading:6708163-Cells, Cultured,
pubmed-meshheading:6708163-Chemical Phenomena,
pubmed-meshheading:6708163-Chemistry,
pubmed-meshheading:6708163-Cross Reactions,
pubmed-meshheading:6708163-Hybridomas,
pubmed-meshheading:6708163-Immunization,
pubmed-meshheading:6708163-Macrophages,
pubmed-meshheading:6708163-Mice,
pubmed-meshheading:6708163-Mice, Inbred BALB C,
pubmed-meshheading:6708163-Radioimmunoassay,
pubmed-meshheading:6708163-Resorcinols,
pubmed-meshheading:6708163-Sheep,
pubmed-meshheading:6708163-Spleen,
pubmed-meshheading:6708163-Zeranol
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pubmed:year |
1984
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pubmed:articleTitle |
Preparation and properties of monoclonal antibodies to the anabolic agent zeranol.
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pubmed:publicationType |
Journal Article
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