Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-4-27
pubmed:abstractText
Sputum samples from patients with chronic bronchitis were assayed for histamine content using a bioassay, and/or fluorometric assay and single or double radioenzymatic techniques. Comparison showed that all the assays produced significant differences in the measurement of sputum histamine. Similarly, it was observed that the assays all showed significant differences in the measurement of histamine released from human basophils. In general the fluorometric assay give the highest values for histamine in sputum and the double-isotope radioenzymatic assay the lowest. In contrast, no differences were found between the bioassay, fluorometric assay and the single-isotope radioenzymatic assay for the measurement of histamine added to Tyrode's solution. These results suggest that in biological samples some of the assays may additionally be measuring metabolites of histamine or other components present in the samples and underline the need to measure the levels of histamine and its metabolites separately in sputum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0009-9090
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
A comparison of the available methods for the measurement of histamine in sputum.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't