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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1977-6-11
|
| pubmed:abstractText |
A number of salt solutions were tested for their ability to replace 2 X SSC in the production of C-bands with the so-called CT technique. Those containing monovalent cations, except for the ones with Ag+ ions, were capable of inducing C-bands in Ba(OH)2-pretreated human chromosomes. Cesium cations were the most effective in this respect. In contrast to the effect of the monovalent cations studied, di- or polyvalent cations (and Ag+ cations) were incapable of producing C-bands, but appeared to stabilize the Ba(OH)2-induced reverse-banding pattern, This stabilizing effect was obtainable with relatively low cation concentrations and persisted even when C-band-producing cations were present at the same time. The experiments clearly show that the cations present during salt incubations, generally used in the CT- and several other banding methods, can exert a pronounced influence on chromosome behavior.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0301-0171
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2-12
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1977
|
| pubmed:articleTitle |
The effect of cations on C-band formation in human chromosomes.
|
| pubmed:publicationType |
Journal Article
|