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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-3-29
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pubmed:abstractText |
Four transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-174105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-386835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4200179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4216403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4301313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4303477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4613857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4737663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-6081701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-6253151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-6260624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-6283371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-6286853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-664220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-7150405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-726266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-7350544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-7441821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-886304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-912752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-913604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-919348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-947550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6700581-988194
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
110-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6700581-Adenoviruses, Human,
pubmed-meshheading:6700581-Animals,
pubmed-meshheading:6700581-Base Sequence,
pubmed-meshheading:6700581-Cell Division,
pubmed-meshheading:6700581-Cell Line,
pubmed-meshheading:6700581-Cell Transformation, Viral,
pubmed-meshheading:6700581-DNA, Viral,
pubmed-meshheading:6700581-Embryo, Mammalian,
pubmed-meshheading:6700581-Humans,
pubmed-meshheading:6700581-Kidney,
pubmed-meshheading:6700581-Mice,
pubmed-meshheading:6700581-Neoplasms, Experimental,
pubmed-meshheading:6700581-Viral Proteins
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pubmed:year |
1984
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pubmed:articleTitle |
Isolation and characterization of four adenovirus type 12-transformed human embryo kidney cell lines.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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