Linked Life Data

6686787

Source:http://linkedlifedata.com/resource/pubmed/id/6686787

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-4-12
pubmed:abstractText
The histochemical localization of plasminogen activator (PA) and the level of the translatable mRNA species coding for active PA were analyzed during ontogenesis of normal and of irradiation-agranulated rat cerebellum. Autoradiographic localization of PA activity was performed by plasminogen-dependent fixation of [125I]fibrin degradation products to frozen sections of developing rat cerebellum. Both the immature external and the adult internal granular layers were intensely labeled, in addition to labeling of meninges. In the irradiation-aggranulated cerebellum, PA labeling could be observed in residual granular neurons which went through their final division prior to the irradiation protocol. The concentration of the mRNA species directing the synthesis of catalytically active PA (PAmRNA) was monitored by an in ovo bioassay, using Xenopus oocytes as a translation system. A major species of 80,000 and a minor species of 50,000 apparent molecular weight of active PA were translated by mRNA from either control or X-irradiated cerebellum throughout ontogenesis. These could be detected by electrophoretic analysis of extracts and incubation media of microinjected oocytes. Both the content and the concentration of PAmRNA were found to be the highest at the stage of cerebellar development when granular neurons proliferate and migrate. These observations suggest that a major portion of the PA activity in the rat cerebellum is synthesized and localized in granular neurons through cerebellar ontogenesis, and that PA activity in the developing cerebellum is largely determined by the level of translatable mRNA coding for this enzyme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:volume
313
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-58
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Plasminogen activator in the developing rat cerebellum: biosynthesis and localization in granular neurons.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't