Linked Life Data

6667927

Source:http://linkedlifedata.com/resource/pubmed/id/6667927

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1984-3-30
pubmed:abstractText
At low ionic strength (0.05 M) the sedimentation coefficient of monomeric plasma fibronectin was found to vary from 8S, at pH 3 and 11, to 13.5S at neutral pH. The lower s20,w value indicates a stretched arrangement of the value indicates a stretched arrangement of the two arms of the molecule which was observed in most electron microscopic studies. The higher value is consistent with a still very asymmetric but more condensed shape, which is probably brought about by back-folding and interactions between chain segments of different net charge. A model of this internal association is based on the finding that segments of rather different isoelectric points alternate along the fibronectin chains. A similar pH dependence was observed for a 140-kDa fragment from the middle region of fibronectin which carries segments of low and high isoelectric points at its ends. At high ionic strength (0.35 M) the pH dependence of the sedimentation coefficients was less pronounced and intermediate s20,w values were found. This is expected when both repulsive and attractive interactions are weakened by the electrolyte. It was verified by circular dichroism spectra that the protein was not denatured at pH 3 or 11. Thermal transition curves revealed a destabilization at pH 3 but the thermal denaturation occurred well above 20 degrees C at which the pH dependence of the solution shape was studied.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0018-4888
pubmed:author
pubmed:issnType
Print
pubmed:volume
364
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1795-804
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Shape and stability of fibronectin in solutions of different pH and ionic strength.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't