Linked Life Data

6645599

Source:http://linkedlifedata.com/resource/pubmed/id/6645599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1984-1-7
pubmed:abstractText
Long term murine bone marrow cultures maintain orderly granulopoiesis for several months. Assay of cell free supernatant obtained at weekly feedings from control cultures demonstrated endogeneously produced acidic isoferritin inhibitory activity. Addition of 10(9) M acidic isoferritin inhibitory activity to long term cultures gave a trend toward fewer nonadherent cells and fewer colony forming units-granulocyte/macrophage (CFU-GM) per flask, but this was not statistically significant. Media conditioned by each fraction of velocity sedimentation separated non-adherent cells gave two discrete peaks of inhibition at 5.6 and 6.8-9 mm/h, respectively. Acidic isoferritin inhibitory activity was restricted to the sharp peak at 5.6 mm/h. Media conditioned by each fraction of velocity sedimentation separated adherent layer cells did not contain assayable acidic isoferritin inhibitory activity. Tests for an endogeneously produced soluble inactivator of acidic isoferritin inhibitory activity were negative. The constitutive production and release of acidic isoferritin inhibitory activity by a restricted population of cells within long term marrow cultures supports the concept of this molecule as a normal hematopoietic regulator.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0145-2126
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
581-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Acidic isoferritin inhibitory activity: a normal granulopoietic regulator within long term mouse bone marrow cultures.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't