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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1984-1-27
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pubmed:abstractText |
A serum lectin specific for mannose and N-acetylglucosamine residues was isolated from human serum to near homogeneity mainly by affinity chromatography on a column of Sepharose 4B-mannan. The lectin, called mannan-binding protein, was a glycine-rich protein with an apparent molecular size of approximately 600,000 daltons, and had a subunit structure consisting of a single component with an apparent molecular weight of 31,000. Binding of the isolated lectin to 125I-labeled mannan was dependent upon the presence of Ca2+, proportional to the protein added, and a reversible and saturable process. Scatchard plot analysis of binding data indicated the presence of a binding site with a dissociation constant of 2.3 X 10(-9) M and a maximum capacity of 4.3 pmol of 125I-labeled mannan per microgram of protein (2.6 mol of mannan per mol of the protein). The mannan-binding protein, is different from C-reactive protein (CRP) and amyloid P-component (SAP), both of which are serum components known to bind polysaccharides in the presence of Ca2+. A distinct binding activity toward mannan which did not require Ca2+ was attributed to immunoglobulins (IgG).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collectins,
http://linkedlifedata.com/resource/pubmed/chemical/Mannans,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
937-47
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:6643429-Amino Acids,
pubmed-meshheading:6643429-Calcium,
pubmed-meshheading:6643429-Carrier Proteins,
pubmed-meshheading:6643429-Collectins,
pubmed-meshheading:6643429-Humans,
pubmed-meshheading:6643429-Kinetics,
pubmed-meshheading:6643429-Mannans,
pubmed-meshheading:6643429-Molecular Weight,
pubmed-meshheading:6643429-Sialic Acids,
pubmed-meshheading:6643429-Structure-Activity Relationship
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pubmed:year |
1983
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pubmed:articleTitle |
Isolation and characterization of a mannan-binding protein from human serum.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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