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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1983-8-11
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pubmed:abstractText |
A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethyl-silyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 +/- 2.0 pg/ml.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0034-5164
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
73-86
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6575411-Adult,
pubmed-meshheading:6575411-Chromatography, Thin Layer,
pubmed-meshheading:6575411-Dinoprostone,
pubmed-meshheading:6575411-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:6575411-Humans,
pubmed-meshheading:6575411-Middle Aged,
pubmed-meshheading:6575411-Prostaglandins E
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pubmed:year |
1983
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pubmed:articleTitle |
Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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