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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1983-7-8
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pubmed:abstractText |
Rats fed a K-deficient diet exhibit accelerated kidney growth and enhanced activity of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (G3PD; D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase, EC 1.2.1.12). Cultures of renal epithelial cells (BSC-1 line) served as a model for this phenomenon because a reduction in the medium K concentration from 5.4 to 3.2 mM resulted in augmented growth and increased G3PD activity. Mixing the soluble supernatant fraction from cells grown in low-K medium (3.2 mM) with that from cells grown in control medium indicated that the cytosol of low-K cells contained a positive modifier of G3PD activity. Appearance of modifier activity that occurred within 1 hr after exposure of cells to low-K medium was blocked by cycloheximide but not by actinomycin D. Modifier activity was also observed in mouse fibroblasts stimulated by low-K medium and in proliferating renal medullary tissue from rats fed a K-deficient diet. A single protein that contained G3PD-stimulating activity was isolated from the soluble supernatant of BSC-1 cells exposed to low-K medium. This protein was not detectable in control cells. The material yielded a single band on NaDodSO4/polyacrylamide gel electrophoresis with an apparent Mr of 62,000. These results suggest that a new protein with the capacity to stimulate G3PD activity appears in the cytosol during the initiation of cell growth.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-13298683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-13475614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-137907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-14334364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-146038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-146879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-175213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-291975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-303774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-304448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-4207235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-4602166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-470344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-509519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-56781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-6446316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-6935677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-7176327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-7447472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-82969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-830994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-839728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6574463-902318
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2941-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6574463-Animals,
pubmed-meshheading:6574463-Cell Division,
pubmed-meshheading:6574463-Cells, Cultured,
pubmed-meshheading:6574463-Cytosol,
pubmed-meshheading:6574463-Epithelial Cells,
pubmed-meshheading:6574463-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:6574463-Glycolysis,
pubmed-meshheading:6574463-Kidney,
pubmed-meshheading:6574463-Mitogens,
pubmed-meshheading:6574463-Potassium,
pubmed-meshheading:6574463-Proteins,
pubmed-meshheading:6574463-Rats
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pubmed:year |
1983
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pubmed:articleTitle |
Appearance of a cytosolic protein that stimulates glyceraldehyde-3-phosphate dehydrogenase activity during initiation of renal epithelial cell growth.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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