Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1978-9-1
pubmed:abstractText
Both silkworm nuclease and nuclease O of Aspergillus oryzae mycelia hydrolyse DNA endolytically to di- and trinucleotides terminating in 5'-phosphate. These oligonucleotides were fractionated first be DEAE-cellulose chromatography with 7 M urea into the respective isoplithic groups and then analysed for the composition and isomerism: each group was labeled 5'-terminally by the [gamma-32P]ATP-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-dimensionally for the di- and trinucleotides mixtures, respectively, followed by elution and digestion with snake venom exonuclease. Both nucleases gave rather similar simple maps in which all sixteen dinucleotides and almost all the possible trinucleotides were identified, indicating their random mode of actions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
518
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
381-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Mapping of deoxydi- and trinucleotides liberated by the action of endonucleases from the silkworm and Aspergillis oryzae.
pubmed:publicationType
Journal Article