Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1978-7-24
pubmed:abstractText
Cobra venom factor (CoF), the anticomplementary protein in Naja naja cobra venom, is usually purified by sequential ion exchange and gel filtration chromatography. CoF prepared in this manner contains small but significant quantities of phospholipase A2 activity. This acyl hydrolase activity can be simply and efficiently removed on a large scale by treatment of CoF with p-bromophenacyl bromide (BPB), an irreversible modifier of the histidine residue in the active site of phospholipase A2. BPB treatment does not alter the anticomplementary activity of CoF. In vivo experiments utilizing intratracheal injections of control and BPB-treated CoF, as well as pure phospholipase A2, revealed that contaminating phospholipase A2, and not the anticomplementary protein, was responsible for the observed acute neutrophil-associated lung injury. However, phospholipase A2 had no effect on the hypotensive and thrombocytopenic effects of CoF infected intravenously into rabbits. Depletion of circulating C3-C9 by intraperitoneal injections of CoF was not altered by removal of phospholipase A2 activity with BPB.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-1005744, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-1086222, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-1104750, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-1167871, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-1194273, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-126034, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-13699041, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-14964, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-19461, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-266715, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-367962, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4246566, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4459260, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4627511, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4698263, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4734404, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4834789, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-4981797, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-5120401, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-790690, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-849467, http://linkedlifedata.com/resource/pubmed/commentcorrection/655262-992719
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
517-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Phospholipase A2 contamination of cobra venom factor preparations. Biologic role in complement-dependent in vivo reactions and inactivation with p-bromophenacyl bromide.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.