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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0070479,
umls-concept:C0071629,
umls-concept:C0105810,
umls-concept:C0205250,
umls-concept:C0392747,
umls-concept:C0441655,
umls-concept:C0678594,
umls-concept:C1024032,
umls-concept:C1143198,
umls-concept:C1524075,
umls-concept:C1554963,
umls-concept:C1627358,
umls-concept:C2349975,
umls-concept:C2700384
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pubmed:dateCreated |
1984-10-18
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pubmed:abstractText |
Pestalotia sp. 815, a newly isolated fungus, produces extracellularly a highly (1----6)-branched (1----3)-beta-D-glucan in high yield when grown in a D-glucose-containing medium. This extracellular glucan, designated "Pestalotan", has [alpha]25D-0.1 degree (c 0.5, M NaOH) and a molecular weight greater than 2 X 10(6). Chemical and enzymic studies indicated that pestalotan has a very highly branched structure containing a back-bone chain of beta-D-(1----3)-linked D-glucosyl residues, and three out of five D-glucosyl residues are substituted at O-6, mostly with single D-glucosyl groups, and a very few with short beta-(1----6)-linked oligosaccharide units. This D-glucan becomes water-insoluble after isolation from the culture filtrate followed by dehydration, and shows moderate growth-inhibitory activities against mouse-implanted tumors. However, when the D-glucosyl groups of the side chains were modified by periodate oxidation and borohydride reduction, the resulting, still water-insoluble, D-glucan polyol exhibited potent antitumor activities, confirming that the attachment of many polyhydroxy groups to the beta-D-(1----3)-linked D-glucan back-bone gives a remarkable enhancement effect on the antitumor activity of the branched D-glucan. Prolonged treatment of the D-glucan polyol by ultrasonic irradiation afforded a low-molecular-weight D-glucan polyol (SD-pestalotan polyol), without alteration of its chemical structure. The water-soluble, SD-pestalotan polyol, having a molecular weight of 4.7 X 10(5), exhibited remarkable antitumor activities against both allogeneic and syngeneic, mouse-implanted tumors, at small dosages (1-5 mg/kg for 10 days) by intraperitoneal administration. A comparison of values of the molecular weight of SD-pestalotan polyol, estimated by 3-MPa l.c. for the aqueous solution and the dimethyl sulfoxide solution, strongly suggested that the D-glucan polyol must form a triple-chain conformation in aqueous solution.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0008-6215
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-27
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6547877-Animals,
pubmed-meshheading:6547877-Antineoplastic Agents,
pubmed-meshheading:6547877-Carcinoma, Ehrlich Tumor,
pubmed-meshheading:6547877-Culture Media,
pubmed-meshheading:6547877-Drug Evaluation, Preclinical,
pubmed-meshheading:6547877-Fungi,
pubmed-meshheading:6547877-Glucans,
pubmed-meshheading:6547877-Mice,
pubmed-meshheading:6547877-Mice, Inbred C3H,
pubmed-meshheading:6547877-Mice, Inbred ICR,
pubmed-meshheading:6547877-Sarcoma 180
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pubmed:year |
1984
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pubmed:articleTitle |
Structure of pestalotan, a highly branched (1----3)-beta-D-glucan elaborated by Pestalotia sp. 815, and the enhancement of its antitumor activity by polyol modification of the side chains.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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