Linked Life Data

6547057

Source:http://linkedlifedata.com/resource/pubmed/id/6547057

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-7-16
pubmed:abstractText
A new method for immobilizing protein on the surface of liposomes is described. Inclusion of N-(p-aminophenyl) stearylamide in the lipid composition of vesicles resulted in liposomes that could be 'activated' by diazotization with NaNO2/HCl, and subsequently coupled with protein. Using this method 39.7 +/- 7.5 micrograms egg albumin/mumol phospholipid has been coupled to multilamellar vesicles composed of phosphatidylcholine, cholesterol, and N-(p-aminophenyl) stearylamide in a molar ratio of 15:75:1.1. Furthermore, when the immunologic response of mice to egg albumin that was encapsulated in, nonspecifically absorbed, or covalently linked to liposomes was investigated, only the covalent protein-liposome conjugates elicited pronounced and sustained elevations in antibody titers. These results suggest that the immunoadjuvant effects of liposomes can be maximized by covalently linking protein antigens to their surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
772
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-94
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Immunologic response to protein immobilized on the surface of liposomes via covalent azo-bonding.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.