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rdf:type |
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lifeskim:mentions |
umls-concept:C0002518,
umls-concept:C0006556,
umls-concept:C0017837,
umls-concept:C0032136,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035696,
umls-concept:C0332256,
umls-concept:C0441513,
umls-concept:C0679058,
umls-concept:C1527177,
umls-concept:C1547699,
umls-concept:C1706793,
umls-concept:C1711351,
umls-concept:C1880022,
umls-concept:C2700640
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pubmed:issue |
1
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pubmed:dateCreated |
1984-6-18
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pubmed:databankReference |
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pubmed:abstractText |
Free polyribosomal poly(A)-containing RNA isolated from normal rat liver was used to prepare a complementary DNA plasmid library in the Pst1 site of the plasmid pAT153 . A plasmid pGSTr155 complementary to mRNA coding for a glutathione transferase Ya subunit was selected by differential hybridization in situ and preliminary characterization was performed by hybrid-selected mRNA translation, immunoprecipitation and polyacrylamide-gel electrophoresis of the product synthesized in vitro. The nucleotide sequence of the complementary DNA contained within pGSTr155 was determined and shown to contain a single open reading frame corresponding to the first 129 amino acids of the N-terminus of the Ya subunit and a further 63 nucleotides upstream of the initiating methionine codon.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-1008846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-405047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-414220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-4519026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-493130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-518553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-5432061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-597256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6166921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6187739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6273441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6285305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6292839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6339228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6617979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6653549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-667061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6896146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-6997135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-7067918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-7107616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-7140737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-7306038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6547043-823012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
219
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
223-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6547043-Amino Acid Sequence,
pubmed-meshheading:6547043-Animals,
pubmed-meshheading:6547043-Base Sequence,
pubmed-meshheading:6547043-DNA,
pubmed-meshheading:6547043-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6547043-Glutathione Transferase,
pubmed-meshheading:6547043-Liver,
pubmed-meshheading:6547043-Male,
pubmed-meshheading:6547043-Plasmids,
pubmed-meshheading:6547043-RNA, Messenger,
pubmed-meshheading:6547043-RNA, Ribosomal,
pubmed-meshheading:6547043-Rats,
pubmed-meshheading:6547043-Rats, Inbred Strains,
pubmed-meshheading:6547043-Recombination, Genetic,
pubmed-meshheading:6547043-Transcription, Genetic
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pubmed:year |
1984
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pubmed:articleTitle |
Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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