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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0023884,
umls-concept:C0024188,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0032136,
umls-concept:C0035696,
umls-concept:C0162326,
umls-concept:C0332120,
umls-concept:C0441513,
umls-concept:C0456205,
umls-concept:C0591833,
umls-concept:C0851285,
umls-concept:C1521739,
umls-concept:C1527177,
umls-concept:C1550605,
umls-concept:C1705542
|
| pubmed:issue |
2
|
| pubmed:dateCreated |
1984-3-5
|
| pubmed:abstractText |
Mouse liver mRNA that was enriched in sequences coding for ATP-citrate lyase by polysome immunoadsorption was used as a template for cDNA synthesis. Double-stranded cDNA sequences were inserted into the plasmid pBR322 and cloned in Escherichia coli RR1. Twenty-seven plasmids containing putative cDNA sequences for ATP-citrate lyase were identified by differential hybridization with single-stranded 32P-cDNAs synthesized from immunopurified mRNA, sucrose gradient-purified ATP-citrate lyase mRNA, and mRNA isolated from the livers of mice that were nutritionally induced or de-induced for ATP-citrate lyase biosynthesis. A subgroup of five recombinant plasmids was characterized further in hybridization-selection experiments. Each of these plasmids positively selected ATP-citrate lyase mRNA as determined by in vitro translation and specific immunoprecipitation. The length of ATP-citrate lyase mRNA was estimated to be 4900 bases in a Northern blot analysis. A 32P-cDNA probe derived from a 1500-base pair insert was used to investigate the basis for the 20-30-fold induction of ATP-citrate lyase that occurs when starved animals are fed a high carbohydrate/low fat diet. Dot-blot hybridization analysis disclosed that the relative content of liver ATP-citrate lyase mRNA increased 25-fold after 15 h of refeeding, indicating that the synthesis of the lipogenic enzyme is controlled at a pretranslational level in the nutritional paradigm.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
259
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1201-5
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6546379-ATP Citrate (pro-S)-Lyase,
pubmed-meshheading:6546379-Animals,
pubmed-meshheading:6546379-Base Sequence,
pubmed-meshheading:6546379-Cloning, Molecular,
pubmed-meshheading:6546379-DNA,
pubmed-meshheading:6546379-Food,
pubmed-meshheading:6546379-Liver,
pubmed-meshheading:6546379-Mice,
pubmed-meshheading:6546379-Mice, Inbred C57BL,
pubmed-meshheading:6546379-Plasmids,
pubmed-meshheading:6546379-RNA, Messenger,
pubmed-meshheading:6546379-Starvation,
pubmed-meshheading:6546379-Templates, Genetic
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Cloning of cDNA sequences for murine ATP-citrate lyase. Construction of recombinant plasmids using an immunopurified mRNA template and evidence for the nutritional regulation of ATP-citrate lyase mRNA content in mouse liver.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|