| pubmed-article:6543229 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0597694 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0029923 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0162768 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0037081 | lld:lifeskim |
| pubmed-article:6543229 | lifeskim:mentions | umls-concept:C0080141 | lld:lifeskim |
| pubmed-article:6543229 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6543229 | pubmed:dateCreated | 1985-4-4 | lld:pubmed |
| pubmed-article:6543229 | pubmed:abstractText | The intramolecular signals for chicken ovalbumin secretion were examined by producing mutant proteins in Xenopus oocytes. An ovalbumin complementary DNA clone was manipulated in vitro, and constructs containing altered protein-coding sequences and either the simian virus 40 (SV40) early promoter or Herpes simplex thymidine kinase promoter, were microinjected into Xenopus laevis oocytes. The removal of the eight extreme N-terminal amino acids of ovalbumin had no effect on the segregation of ovalbumin with oocyte membranes nor on its secretion. A protein lacking amino acids 2 to 21 was sequestered in the endoplasmic reticulum but remained strongly associated with the oocyte membranes rather than being secreted. Removal of amino acids 231 to 279, a region previously reported to have membrane-insertion function, resulted in a protein that also entered the endoplasmic reticulum but was not secreted. Hybrid proteins containing at their N terminus amino acids 9 to 41 or 22 to 41 of ovalbumin fused to the complete chimpanzee alpha-globin polypeptide were also sequestered by oocyte membranes. We conclude that the ovalbumin "signal" sequence is internally located within amino acids 22 to 41, and we speculate that amino acids 9 to 21 could be important for the completion of ovalbumin translocation through membranes. | lld:pubmed |
| pubmed-article:6543229 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6543229 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6543229 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6543229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6543229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6543229 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6543229 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6543229 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6543229 | pubmed:issn | 0022-2836 | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:JacksonDD | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:ColmanAA | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:KriegPP | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:WallisEE | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:TabeLL | lld:pubmed |
| pubmed-article:6543229 | pubmed:author | pubmed-author:StrachanRR | lld:pubmed |
| pubmed-article:6543229 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6543229 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:6543229 | pubmed:volume | 180 | lld:pubmed |
| pubmed-article:6543229 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6543229 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6543229 | pubmed:pagination | 645-66 | lld:pubmed |
| pubmed-article:6543229 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:6543229 | pubmed:meshHeading | pubmed-meshheading:6543229-... | lld:pubmed |
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| pubmed-article:6543229 | pubmed:meshHeading | pubmed-meshheading:6543229-... | lld:pubmed |
| pubmed-article:6543229 | pubmed:meshHeading | pubmed-meshheading:6543229-... | lld:pubmed |
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| pubmed-article:6543229 | pubmed:meshHeading | pubmed-meshheading:6543229-... | lld:pubmed |
| pubmed-article:6543229 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6543229 | pubmed:articleTitle | Segregation of mutant ovalbumins and ovalbumin-globin fusion proteins in Xenopus oocytes. Identification of an ovalbumin signal sequence. | lld:pubmed |
| pubmed-article:6543229 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6543229 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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