Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-3-6
pubmed:abstractText
Chemical modification of amino acid residues with phenylglyoxal, N-ethylmaleimide and diethyl pyrocarbonate indicated that at least one residue each of arginine, cysteine and histidine were essential for the activity of sheep liver serine hydroxymethyltransferase. The second-order rate constants for inactivation were calculated to be 0.016 mM-1 X min-1 for phenylglyoxal, 0.52 mM-1 X min-1 for N-ethylmaleimide and 0.06 mM-1 X min-1 for diethyl pyrocarbonate. Different rates of modification of these residues in the presence and in the absence of substrates and the cofactor pyridoxal 5'-phosphate as well as the spectra of the modified protein suggested that these residues might occur at the active site of the enzyme.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-1009937, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-1164432, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-1239280, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-14109201, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-190679, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-22021, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-388184, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-4738233, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-4741279, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-5561469, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-6268170, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-656497, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-7036682, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-7217053, http://linkedlifedata.com/resource/pubmed/commentcorrection/6525172-921937
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
224
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
703-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Identification of active-site residues of sheep liver serine hydroxymethyltransferase.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't