Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1985-2-28
pubmed:abstractText
The interaction of the galactocerebroside, N-palmitoylgalactosylsphingosine (NPGS), with cholesterol has been studied by differential scanning calorimetry (DSC) and x-ray diffraction. Thermal and structural studies demonstrate complex behavior characterized by two endothermic transitions: transition I (TI approximately equal to 50-60 degrees C) corresponding to an NPGS-cholesterol bilayer gel----bilayer liquid crystal transition II (TII where TI less than TII less than TNPGS) corresponding to an NPGS bilayer crystal (stable E form)----bilayer liquid crystal transition. For mixtures containing from 6 to 80 mol % cholesterol, x-ray diffraction studies at 22 degrees C (T less than TI) indicate two separate lamellar phases; an NPGS crystal bilayer phase and a cholesterol monohydrate phase. For cholesterol concentrations less than 50 mol % at TI less than T less than TII, NPGS-cholesterol liquid crystal bilayer and excess NPGS crystal bilayer phases are observed. For greater than 50 mol % cholesterol concentrations at these temperatures, an excess cholesterol monohydrate phase coexists with the NPGS-cholesterol liquid crystal bilayers. At T greater than TII, complete NPGS-cholesterol miscibility is only observed for less than 50 mol % cholesterol concentrations, whereas at greater than 50 mol % cholesterol an excess cholesterol phase is present. The solid phase immiscibility of cerebroside and cholesterol at low temperatures is suggested to result from preferential NPGS-NPGS associations via hydrogen bonding. The unique thermal and structural behavior of NPGS-cholesterol dispersions is contrasted with the behavior of cholesterol-phosphatidycholine and cholesterol-sphingomyelin bilayers. Thermal and structural studies of NPGS in dipalmitoylphosphatidylcholine (DPPC)/cholesterol (1:1, molar ratio) bilayers have been performed. For dispersions containing less than 20 mol % NPGS at 22 degrees C there are no observable calorimetric transitions and x-ray diffraction studies indicate complete lipid miscibility. At greater than 20 mol % NPGS, a high temperature transition is observed that is shown by x-ray diffraction studies to be due to an excess NPGS crystal bilayer----liquid crystal bilayer transition. Complete miscibility of NPGS in DPPC/cholesterol bilayers is observed at T greater than TNPGS. The properties of NPGS/DPPC/cholesterol bilayers are discussed in terms of the lipid composition of the myelin sheath.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-141809, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-143963, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-161835, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-211824, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-4352068, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-435470, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-435481, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-458269, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-4818830, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-486526, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-4905514, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5063098, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5168781, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5283387, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5343454, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-534624, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5680287, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-5689845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-581060, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-581743, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-583131, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-6035283, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-6626552, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-6688367, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-6897624, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7063872, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7082645, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7104317, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7138808, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7260307, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7306486, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7317370, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7359142, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7407065, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-7452300, http://linkedlifedata.com/resource/pubmed/commentcorrection/6518252-991384
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
695-707
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Interaction of cholesterol with galactocerebroside and galactocerebroside-phosphatidylcholine bilayer membranes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.