Linked Life Data

6501423

Source:http://linkedlifedata.com/resource/pubmed/id/6501423

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1985-1-17
pubmed:abstractText
A preparation of rat liver microsomes containing 70% of the total cellular endoplasmic reticulum (ER) membranes was subfractionated by isopycnic density centrifugation. Twelve subfractions of different ribosome content ranging in density from 1.06 to 1.29 were obtained and analyzed with respect to marker enzymes, RNA, and protein content, as well as the capacity of these membranes to bind 80S ribosomes in vitro. After removal of native polysomes from these microsomal subfractions by puromycin in a buffer of high ionic strength their capacity to rebind 80S ribosomes approached levels found in the corresponding native membranes before ribosome stripping. This indicates that in vitro rebinding of ribosomes occurs to the same sites occupied in the cell by membrane-bound polysomes. Microsomes in the microsomal subfractions were also tested for their capacity to effect the translocation of nascent secretory proteins into the microsomal lumen utilizing a rabbit reticulocyte translation system programmed with mRNA coding for the precursor of human placental lactogen. Membranes from microsomes with the higher isopycnic density and a high ribosome content showed the highest translocation activity, whereas membranes derived from smooth microsomes had only a very low translocation activity. These results indicate the membranes of the rough and smooth portions of the endoplasmic reticulum are functionally differentiated so that sites for ribosome binding and the translocation of nascent polypeptides are segregated to the rough domain of the organelle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-1063399, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-13893456, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-14292249, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-271987, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-291894, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-291982, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-293711, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-325648, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-4150489, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-418074, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-4368410, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-4449120, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-4682341, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-4961331, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-5000815, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-560377, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6167823, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6292235, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6292236, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-632254, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-649658, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6501424, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6656642, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-6938958, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7000796, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7035466, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7068631, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7084228, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7088152, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-723266, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7309796, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7309797, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-7364760, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-811671, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-893412, http://linkedlifedata.com/resource/pubmed/commentcorrection/6501423-914845
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2247-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't