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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1976-9-1
|
| pubmed:abstractText |
125I-Iodohydroxybenzylpindolol ([125I] IHYP), a potent beta-adrenergic receptor antagonist, has been used to study beta-adrenergic receptors in rat brain. Binding of [125I] IHYP (30 pM) to a membrane fraction min and dissociation took place with a half time of about 16 min. Phentolamine (10(-4) M) decreased non-receptor binding but it had no effect on the binding of [125I] IHYP to beta-adrenergic receptors in cortex, cerebellum or caudate. In the presence of phentolamine specific binding (defined as binding which was blocked by 0.3 muM dl-propranolol) represented 70-85% of total binding. The binding of [125I] IHYP was inhibited by beta-adrenergic agonists and antagonists. d-Stereoisomers were 2-3 orders of magnitude less potent than the corresponding 1-isomers. The denstiy of [125I] IHYP binding sites was studied in membrane fractions from cerebral cortex, cerebellum, and caudate nucleus by means of Scatchard analysis. The K(D) of [125I] IHYP was similar in the three regions studied, and the density of [125I] IHYP binding sites was approximately 50% greater in the cortex and caudate than in the cerebellum. The Hill coefficient for the binding of [125I] IHYP to membranes from cerebral cortex was 1.02. The properties of the binding of [125I] IHYP are similar to those which would be expected of binding to beta-adrenergic receptors in vitro.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phentolamine,
http://linkedlifedata.com/resource/pubmed/chemical/Pindolol,
http://linkedlifedata.com/resource/pubmed/chemical/Propranolol,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0095-1544
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
149-61
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6494-Adrenergic beta-Agonists,
pubmed-meshheading:6494-Animals,
pubmed-meshheading:6494-Binding, Competitive,
pubmed-meshheading:6494-Binding Sites,
pubmed-meshheading:6494-Brain,
pubmed-meshheading:6494-Brain Stem,
pubmed-meshheading:6494-Caudate Nucleus,
pubmed-meshheading:6494-Cell Membrane,
pubmed-meshheading:6494-Cerebellum,
pubmed-meshheading:6494-Cerebral Cortex,
pubmed-meshheading:6494-Hypothalamus,
pubmed-meshheading:6494-Isoproterenol,
pubmed-meshheading:6494-Kinetics,
pubmed-meshheading:6494-Male,
pubmed-meshheading:6494-Nerve Tissue Proteins,
pubmed-meshheading:6494-Phentolamine,
pubmed-meshheading:6494-Pindolol,
pubmed-meshheading:6494-Propranolol,
pubmed-meshheading:6494-Protein Binding,
pubmed-meshheading:6494-Rats,
pubmed-meshheading:6494-Receptors, Adrenergic,
pubmed-meshheading:6494-Stereoisomerism
|
| pubmed:year |
1976
|
| pubmed:articleTitle |
beta-Adrenergic receptors in rat brain.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|