Linked Life Data

6477601

Source:http://linkedlifedata.com/resource/pubmed/id/6477601

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-10-19
pubmed:abstractText
Light-adapted purple membranes were modified with tetranitromethane by a new light-dependent procedure at pH 5.5 which results in a blue-shifted chromophore absorbing at 530nm. This modification affects two aromatic residues. The modified bacteriorhodopsin's ground state chromophore structure is probed by circular dichroism and resonance raman spectroscopy while its photocycle is studied by laser-flash photolysis in the picosecond, microsecond and millisecond time scale. After nitration, the main findings are 1) Interactions between neighboring chromophores are lost, 2) Modified bacteriorhodopsin contains a conformationally changed chromophore but retains a protonated Schiff's base as evidenced by a resonance raman band at 1652 cm-1, 3) A red-shifted intermediate is formed in less than 10 ps after laser excitation, 4) The decay of the M-intermediate is not significantly affected whereas the rise time of the intermediate is enhanced about two fold. These observations are relevant to the role of aromatic acid residues of the apoprotein in the determination of the chromophoric characteristics in bacteriorhodopsin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0158-5231
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Spectroscopic characterization of nitrated purple membranes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.