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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-9-26
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pubmed:abstractText |
By use of a more sensitive method than standard assays, it is demonstrated that alpha-glycerophosphate dehydrogenase activity can be monitored continuously in rat liver mitochondria; maximum activity was obtained by sonication in the presence of Triton X-100. Vitamin K3 (menadione) seems to enhance the activity of the enzyme. The assay in the presence of menadione is linear over a much greater mitochondrial concentration (up to 250 micrograms of protein in the reaction mixture).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
139
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
319-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6476369-Animals,
pubmed-meshheading:6476369-Enzyme Activation,
pubmed-meshheading:6476369-Glycerolphosphate Dehydrogenase,
pubmed-meshheading:6476369-Male,
pubmed-meshheading:6476369-Mitochondria, Liver,
pubmed-meshheading:6476369-Octoxynol,
pubmed-meshheading:6476369-Phenazines,
pubmed-meshheading:6476369-Polyethylene Glycols,
pubmed-meshheading:6476369-Rats,
pubmed-meshheading:6476369-Rats, Inbred Strains,
pubmed-meshheading:6476369-Sonication,
pubmed-meshheading:6476369-Spectrophotometry,
pubmed-meshheading:6476369-Tetrazolium Salts,
pubmed-meshheading:6476369-Vitamin K
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pubmed:year |
1984
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pubmed:articleTitle |
A sensitive, continuous spectrophotometric method for assaying alpha-glycerophosphate dehydrogenase: activation by menadione.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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