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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1981-11-18
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pubmed:abstractText |
The equilibrium binding of the carcinogens N-hydroxy-N-acetyl-2-amino-fluorene (HAAF) and 4-nitroquinoline-1-oxide (NQO) to phi X174RF DNA have been studied by phase partition techniques. Both molecules bind in a cooperative manner with only a few carcinogen molecules binding to each phi X174RF DNA molecule. The binding data for both HAAF and NQO fit a model in which two carcinogens cluster into a small number of sites--four sites for HAAF and twelve sites for NQO. Phase partition techniques were also used to study the binding of actinomycin D to both calf thymus DNA and poly (dG-dC) . poly (dG-dC) at much lower r values than had been previously reported. These data exhibit humped Scatchard plots which are indicative of cooperative binding; the overall shape of the Scatchard plots are consistent with a model for drug induced allosteric transitions in the DNA structure. The cooperativity in the actinomycin D binding to calf thymus DNA increases with decreasing sodium chloride concentration, suggesting a role for DNA flexibility in allosteric binding.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-1177318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-1237323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-13918810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-353875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-353876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-372825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4107107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4371420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4416620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4509343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4782683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-4906322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-5464945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-6030315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-61763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-6258628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-7005967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-7194043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-728540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-807478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-830680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-904688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-917115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6456451-972227
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3175-86
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6456451-2-Acetylaminofluorene,
pubmed-meshheading:6456451-4-Nitroquinoline-1-oxide,
pubmed-meshheading:6456451-Animals,
pubmed-meshheading:6456451-Bacteriophage phi X 174,
pubmed-meshheading:6456451-Base Composition,
pubmed-meshheading:6456451-Cattle,
pubmed-meshheading:6456451-Chemical Phenomena,
pubmed-meshheading:6456451-Chemistry,
pubmed-meshheading:6456451-DNA,
pubmed-meshheading:6456451-DNA, Viral,
pubmed-meshheading:6456451-Dactinomycin,
pubmed-meshheading:6456451-Hydroxyacetylaminofluorene,
pubmed-meshheading:6456451-Kinetics,
pubmed-meshheading:6456451-Nitroquinolines,
pubmed-meshheading:6456451-Polydeoxyribonucleotides,
pubmed-meshheading:6456451-Thymus Gland
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pubmed:year |
1981
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pubmed:articleTitle |
Equilibrium binding of carcinogens and antitumor antibiotics to DNA: site selectivity, cooperativity, allosterism.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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