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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1981-11-22
|
| pubmed:abstractText |
A variety of approaches, including the use of a monoclonal antibody, have indicated that the lymphokine Interleukin 2 is a necessary and sufficient mediator for initiating cytotoxic T cell differentiation in vitro. Mitogen (Con A, PHA) stimulation of spleen cells and K/D or I region stimulation in primary MLC all led to IL-2 production, as measured by the growth of an IL-2 dependent T cell clone, and to the production of a "helper" factor necessary for cytotoxic T lymphocyte (CTL) differentiation against metabolically inactivated stimulator spleen cells. Several lines of evidence suggested that the IL-2 activity and CTL "helper" activity were due to a single molecular entity. These included the observations that: i) There was a linear relationship (R = 0.99) between IL-2 activity and relative CTL "helper" activity regardless of the preparation used (purified IL-2; MLC, or mitogen-induced supernatants). ii) Absorption of MLC supernatants with T cell "blasts" or with IL-2 dependent T cell lines concomitantly removed both IL-2 and CTL "helper" activities. iii) IL-2 activity purified by isoelectric focusing, or by polyacrylamide gel electrophoresis, displayed CTL "helper" activity. iv) Both CTL "helper" and IL-2 activities were neutralized by a monoclonal antibody directed against IL-2.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
127
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1323-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6456304-Absorption,
pubmed-meshheading:6456304-Animals,
pubmed-meshheading:6456304-Antibodies, Monoclonal,
pubmed-meshheading:6456304-Cell Differentiation,
pubmed-meshheading:6456304-Cell Line,
pubmed-meshheading:6456304-Concanavalin A,
pubmed-meshheading:6456304-Cytotoxicity, Immunologic,
pubmed-meshheading:6456304-Interleukin-2,
pubmed-meshheading:6456304-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:6456304-Lymphokines,
pubmed-meshheading:6456304-Male,
pubmed-meshheading:6456304-Mice,
pubmed-meshheading:6456304-Mice, Inbred AKR,
pubmed-meshheading:6456304-Mice, Inbred BALB C,
pubmed-meshheading:6456304-Mice, Inbred C57BL,
pubmed-meshheading:6456304-Mice, Inbred CBA,
pubmed-meshheading:6456304-Mice, Inbred DBA,
pubmed-meshheading:6456304-Receptors, Antigen, T-Cell,
pubmed-meshheading:6456304-T-Lymphocytes
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
The role of interleukin-2 (IL-2) in the differentiatin of cytotoxic T cells: the effect of monoclonal anti-IL-2 antibody and absorption with IL-2 dependent T cell lines.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|