Linked Life Data

6453588

Source:http://linkedlifedata.com/resource/pubmed/id/6453588

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-7-23
pubmed:abstractText
1. Recycling of metabolites between fructose 6-phosphate and triose phosphates has been investigated in isolated hepatocytes by the randomization of carbon between C((1)) and C((6)) of glucose formed from [1-(14)C]galactose. 2. Randomization of carbon atoms was regularly observed with hepatocytes isolated from fed rats and was then little influenced by the concentration of glucose in the incubation medium. It was decreased by about 50% in the presence of glucagon. 3. Randomization of carbon atoms by hepatocytes isolated from starved rats was barely detectable at physiological concentrations of glucose in the incubation medium, but was greatly increased with increasing glucose concentrations. It was nearly completely suppressed by glucagon. These large changes can be attributed to parallel variations in the activity of phosphofructokinase. 4. The main factors that appear to control the activity of phosphofructokinase under these experimental conditions are the concentration of fructose 6-phosphate, the concentration of fructose 1,6-bisphosphate and also the affinity of the enzyme for fructose 6-phosphate. 5. The affinity of phosphofructokinase for fructose 6-phosphate was diminished by incubation of the cells in the presence of glucagon and also by filtration of an extract of hepatocytes through Sephadex G-25 and by purification of the enzyme. When assayed at 0.25 or 0.5mm-fructose 6-phosphate, the activity of phosphofructokinase present in a liver Sephadex filtrate was increased by a low-molecular-weight effector, which could be isolated from a liver extract by ultrafiltration, gel filtration or heat treatment, but was rapidly destroyed in trichloroacetic acid, even in the cold. This effector appears to be a highly acid-labile phosphoric ester. Its concentration was greatly increased in hepatocytes incubated in the presence of glucose and was decreased in the presence of glucagon.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-1015825, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-1201008, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-13845757, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-156182, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-157135, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-202464, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-221473, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-237475, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-5158899, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-5966267, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-6272698, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-6453589, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-747652, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453588-962076
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
192
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
887-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Control of the fructose-6-phosphate/fructose 1,6-bisphosphate cycle in isolated hepatocytes by glucose and glucagon. Role of a low-molecular-weight stimulator of phosphofructokinase.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.