Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-12-17
|
pubmed:abstractText |
The binding sites for the allosteric activator, AMP, to glycogen phosphorylase b are described in detail utilizing the more precise knowledge of the native structure obtained from crystallographic restrained least-squares refinement than has hitherto been available. Localized conformational changes are seen at the allosteric effector site that include shifts of between 1 and 2 A for residues Tyr75 and Arg309 and very small shifts for the region of residues 42 to 44 from the symmetry-related subunit. Kinetic studies demonstrate that NADH inhibits the AMP activation of glycogen phosphorylase b. Crystallographic binding studies at 3.5 A resolution show that NADH binds to the same sites on the enzyme as AMP, i.e. the allosteric effector site N, which is close to the subunit-subunit interface, and the nucleoside inhibitor site I, which is some 12 A from the catalytic site. The conformations of NADH at the two sites are different but both conformations are "folded" so that the nicotinamide ring is close (approx. 6 A) to the adenine ring. These conformations are compared with those suggested from solution studies and with the extended conformations observed in the single crystal structure of NAD+ and for NAD bound to dehydrogenases. Possible mechanisms for NADH inhibition of phosphorylase activation are discussed.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/NAD,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylase b,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylases
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0022-2836
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
25
|
pubmed:volume |
170
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
529-65
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6415289-Adenosine Monophosphate,
pubmed-meshheading:6415289-Allosteric Site,
pubmed-meshheading:6415289-Animals,
pubmed-meshheading:6415289-Binding Sites,
pubmed-meshheading:6415289-Crystallography,
pubmed-meshheading:6415289-Enzyme Activation,
pubmed-meshheading:6415289-Kinetics,
pubmed-meshheading:6415289-Macromolecular Substances,
pubmed-meshheading:6415289-Molecular Conformation,
pubmed-meshheading:6415289-NAD,
pubmed-meshheading:6415289-Phosphorylase b,
pubmed-meshheading:6415289-Phosphorylases,
pubmed-meshheading:6415289-Protein Conformation,
pubmed-meshheading:6415289-Rabbits
|
pubmed:year |
1983
|
pubmed:articleTitle |
Comparison of AMP and NADH binding to glycogen phosphorylase b.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|