Linked Life Data

6415175

Source:http://linkedlifedata.com/resource/pubmed/id/6415175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-12-17
pubmed:abstractText
We describe a procedure for determining immunoglobulin isotype and light chain class of murine monoclonal antibodies. Isotype and light chain-specific antibodies are immobilized as dots on a strip of nitrocellulose filter paper. The immobilized antibodies retain binding specificity, and the strips are used in a type of 'sandwich' immunoassay wherein murine monoclonal immunoglobulins bound to the appropriate anti-isotype dot are detected with a peroxidase-conjugated anti-mouse immunoglobulin reagent. The immunodot isotyping assay is specific and sensitive enough to detect mouse immunoglobulin present in ng/ml concentrations. It is as easy or easier to set up and perform than conventional isotyping techniques. It has the added advantages that it greatly conserves antisera reagents and can be performed on hybridoma culture supernates without the need to concentrate, expand, or purify them.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-90
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Immunodot assay for determining the isotype and light chain type of murine monoclonal antibodies in unconcentrated hybridoma culture supernates.
pubmed:publicationType
Journal Article