Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
1983-12-17
|
pubmed:abstractText |
A bacterial factor preventing precipitation of immune complexes was purified from a culture of Streptococcus faecalis (AK strain) by gel filtration on Sephadex G-100 and Bio-Gel A-150m columns. The factor was identified as DNA on the basis of its ultraviolet absorption maximum at 260 nm, hydrolysis by deoxyribonuclease, Mr exceeding 1.5 X 10(8), and positive reaction with diphenylamine. DNA interferes with the immunoassay by preventing precipitation of immune complexes. Only double-stranded DNA and polynucleotides showed this property. Evidently, double-stranded DNA will give false-positive interference with the usual RIA procedures.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0009-9147
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
29
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1912-5
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:6414736-Antigen-Antibody Complex,
pubmed-meshheading:6414736-Chorionic Gonadotropin,
pubmed-meshheading:6414736-Chromatography, Gel,
pubmed-meshheading:6414736-DNA, Bacterial,
pubmed-meshheading:6414736-Enterococcus faecalis,
pubmed-meshheading:6414736-False Positive Reactions,
pubmed-meshheading:6414736-Radioimmunoassay
|
pubmed:year |
1983
|
pubmed:articleTitle |
DNA interference with radioimmunoassay procedures.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|