Linked Life Data

6414477

Source:http://linkedlifedata.com/resource/pubmed/id/6414477

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-11-23
pubmed:abstractText
The role of mucus glycoprotein, lipid and protein in the retardation of hydrogen ion diffusion by dog gastric mucus was investigated. The uniform layer of mucus (1.0 +/- 0.2mm thick), formed on the sintered glass support placed between equimolar (0.155M) solutions of HCl and NaCl, caused a 73% reduction in hydrogen ion diffusion when compared with a layer of NaCl. The rate of hydrogen ion diffusion through purified mucus glycoprotein was similar to that of native mucus, whereas a 30% increase in permeability of glycoprotein to hydrogen ion was observed following its delipidation. Preincubation of the purified mucus glycoprotein with serum albumin or IgA led to an increase in the ability of glycoprotein to retard the diffusion of hydrogen ion. This effect of albumin and IgA occurred at a physiological dose. About 70% decrease in hydrogen ion retardation was obtained following digestion of purified mucus glycoprotein with pepsin, and 74% following its digestion with pronase. The results show that the ability of gastric mucus to retard the diffusion of hydrogen ion depends upon the polymeric structure of undegraded mucus glycoprotein and the extent of interaction of this glycoprotein with lipids, serum albumin, and secretory IgA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1053-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Retardation of hydrogen ion diffusion by gastric mucus constituents: effect of proteolysis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.