Linked Life Data

6394743

Source:http://linkedlifedata.com/resource/pubmed/id/6394743

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1985-2-27
pubmed:abstractText
The USP XX colorimetric assay for the determination of diazotizable substances in hydrochlorothiazide was studied. Colorimetric assay results of hydrochlorothiazide bulk powder and captopril-hydrochlorothiazide combination tablets were found to have a high bias when compared with HPLC. A kinetic study of the diazotization step in the colorimetric assay and extrapolation of the free amine content, i.e., 4-amino-6-chloro-1, 3-benzenedisulfonamide, to time zero provided results which correlated favorably with those obtained by HPLC. The high bias of the colorimetric assay was shown to result from the formation of 4-nitroso derivative of hydrochlorothiazide, which is formed by acid hydrolysis during the diazotization step. Bendroflumethiazide and flumethiazide also showed increasing free amine content when the time of diazotization was increased.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3549
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1545-7
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Mechanism for the high bias in the USP colorimetric assay for diazotizable substances in hydrochlorothiazide.
pubmed:publicationType
Journal Article