Linked Life Data

6392024

Source:http://linkedlifedata.com/resource/pubmed/id/6392024

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
1985-2-15
pubmed:abstractText
A ColE1 hybrid, pB2, carrying the structural gene for the valyl-tRNA synthetase, valS, and the pyrBI operon was isolated from the Clarke-Carbon collection. Strain GRB238 with a temperature-sensitive mutation in the valS gene showed a three-fold increase in valyl-tRNA synthetase activity after transformation with pB2. The valS gene was subcloned into pBR322 after partial Sau3A digestion of pB2. The new plasmid, pHOV1 had a 9.4-kb insert in pBR322 and cells carrying pHOV1 showed an eight-fold increase in specific valyl-tRNA synthetase activity. The localization and direction of transcription of valS in pHOV1 was determined by transposon-directed mutagenesis and analysis of protein synthesis in minicells. The valS gene was shown to be transcribed opposite to the direction of DNA replication, i.e., counterclockwise.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Molecular cloning and characterization of the gene for Escherichia coli valyl-tRNA synthetase.
pubmed:publicationType
Journal Article, Comparative Study