Linked Life Data

6389701

Source:http://linkedlifedata.com/resource/pubmed/id/6389701

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-12-28
pubmed:abstractText
An indirect rosette assay for detecting islet cell surface antibodies (ICSA), the autoantibodies directed to the membranous antigens of islet cells, has been developed. In this assay, Protein A-conjugated sheep red blood cells (SRBC) were used. Both viable and fixed SRBC were available. The results obtained using this new method in insulin-dependent diabetes mellitus (IDDM) patients were not only sensitive, but also correlated with results of the indirect immunofluorescence method currently used for detecting ICSA. Furthermore, in studies of streptozotocin-treated diabetic mice or rats, the courses of ICSA appearance were successively demonstrated by this indirect rosette formation assay. Special advantages of this method are that materials are easily obtained and cost-effective, as the sample volume is small and the number of islet cells required is also small. Assays can be readily performed without a need for radioisotopic or fluorescent equipment. This method provides a reliable laboratory test for diagnosis of autoimmune diabetes mellitus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-80
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Detection of islet cell surface antibodies by an indirect rosette assay, using islet cells and Protein A-labeled sheep red blood cells.
pubmed:publicationType
Journal Article