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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1984-10-24
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pubmed:abstractText |
The shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase, EC 2.5.1.19) has been purified to apparent homogeneity from Aerobacter aerogenes, strain 62-1 (= Klebsiella pneumoniae ATCC 25306). A 3300-fold purification of the enzyme was achieved by ammonium sulfate fractionation, heat precipitation, chromatography on DEAE-cellulose, Sephadex G-75, and cellulose phosphate, and chromatofocusing as the final step. The recovery was 49%. An apparent relative molecular mass of 32400 was determined by calibrated gel filtration, while a single peptide chain of Mr = 42900 was found by sodium dodecyl sulfate/acrylamide gel electrophoresis. The isoelectric point was determined to be at pH 4.6. Two distinct pH optima (pH 5.4 and 6.8) were observed for the enzyme-catalyzed formation of EPSP from phosphoenolpyruvate (PEP) and shikimate 3-phosphate(S3P). For the reverse reaction, the pH optima were 5.6 and 7.6. No evidence for a metal cofactor was found. While the temperature optimum was at 60 degrees C, the activation energies were calculated to be 54.2 kJ/mol for the forward, and 64.1 kJ/mol for the reverse reaction. At low PEP and S3P concentrations, anions acted as activators of EPSP synthase at low concentrations, and as inhibitors at high concentrations. Non-linear Lineweaver-Burk plots were interpreted to result from the activation of EPSP synthase by its anionic substrates. The following dissociation constants were determined for the respective enzyme-substrate complexes: forward reaction: 43 microM (PEP) and 22 microM (S3P); reverse reaction: 1.3 microM (EPSP) and 2.6 mM (Pi). The kinetic patterns indicate a random sequential mechanism for the forward reaction.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-Phosphoshikimate...,
http://linkedlifedata.com/resource/pubmed/chemical/Alkyl and Aryl Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Cations,
http://linkedlifedata.com/resource/pubmed/chemical/Transferases
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
341-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6381056-3-Phosphoshikimate 1-Carboxyvinyltransferase,
pubmed-meshheading:6381056-Alkyl and Aryl Transferases,
pubmed-meshheading:6381056-Anions,
pubmed-meshheading:6381056-Binding Sites,
pubmed-meshheading:6381056-Cations,
pubmed-meshheading:6381056-Chemical Phenomena,
pubmed-meshheading:6381056-Chemistry,
pubmed-meshheading:6381056-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6381056-Hydrogen-Ion Concentration,
pubmed-meshheading:6381056-Isoelectric Focusing,
pubmed-meshheading:6381056-Kinetics,
pubmed-meshheading:6381056-Klebsiella pneumoniae,
pubmed-meshheading:6381056-Molecular Weight,
pubmed-meshheading:6381056-Substrate Specificity,
pubmed-meshheading:6381056-Temperature,
pubmed-meshheading:6381056-Transferases
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pubmed:year |
1984
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pubmed:articleTitle |
5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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