Linked Life Data

6360384

Source:http://linkedlifedata.com/resource/pubmed/id/6360384

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1984-2-14
pubmed:abstractText
Affinity purified antibodies to type I and type II regulatory subunits (RI and RII, respectively) of cAMP-dependent protein kinase were utilized to identify and localize the cAMP receptor proteins in growing vs regressing MCF-7 tumors in nude mice. In the nuclear extracts of growing tumors the RI antibody immunoprecipitated cAMP receptor protein of 47,000 daltons, whereas the RII antibody precipitated 44,000- and 34,000-dalton cAMP receptor proteins. Following estrogen withdrawal, new species of cAMP receptor proteins with molecular weights of 50,000 and 52,000 appeared in the nuclei of regressing tumors. The 50,000- and 52,000-dalton proteins were specifically precipitated by the RII antibody but not by RI antibody. Indirect immunofluorescence revealed that during regression of MCF-7 tumors, the intensity of immunofluorescence of RII antibody crossreacting cAMP binding proteins dramatically increased in the nucleoli whereas the immunofluorescence of RI remained the same. These results suggest a regulatory role of RII in mammary cancer regression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0309-1651
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
937-46
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Appearance of 50,000- and 52,000-dalton cAMP receptor proteins in the nucleoli of regressing MCF-7 human breast cancer upon estrogen withdrawal.
pubmed:publicationType
Journal Article