Linked Life Data

6359962

Source:http://linkedlifedata.com/resource/pubmed/id/6359962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-1-27
pubmed:abstractText
The rate of cell production in the crypt population of the intestinal epithelium has been determined previously. However, the complex geometry of the tissue makes determination of the rate of cell production in the epithelium as a whole, by traditional methods, extremely difficult if not impossible. In this report, stathmokinetic flow cytometry was used for the direct determination of the rate of cell production in the mouse intestinal epithelium. Mice were given an intraperitoneal injection of colcemid (2.5 mg/kg) and killed 25, 45, 65 and 90 min after injection. The percentage of cells with 4N DNA (G2 and M phase cells) in the epithelium at each time interval was determined with flow cytometry. The results were fitted by linear regression and the rate of cell production derived from the slope of the regression line. Thus, the rate of cell production was found to be 1.86% +/- 0.44 (means +/- SE) per hour in the jejunal epithelium and 1.66% +/- 0.48 per hour in the colonic epithelium. The turnover time of the epithelium as a whole was determined from the inverse of the rate of cell production, and was found to be 53.8 h +/- 12.6 in jejunum and 60.2 h +/- 17.3 in colon. Coulter particle counting was used to measure the number of cells in the intestinal epithelium. The number of epithelial cells was found to be 1.90 X 10(7) +/- 3.11 X 10(5) cells per cm2 in the jejunum and 8.98 X 10(6) +/- 8.05 X 10(5) cells per cm2 in the colon.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-276X
pubmed:author
pubmed:issnType
Print
pubmed:volume
207
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Cell production in mouse intestinal epithelium measured by stathmokinetic flow cytometry and Coulter particle counting.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't