Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-11-23
pubmed:abstractText
The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,000) and two smaller proteins (molecular weights, 5,000 and 3,000), and lysed when incubated in medium containing isopropyl-beta-D-thiogalactopyranoside (IPTG). A 400-base-pair lacp-distal fragment derived from the insert in this plasmid was recloned in the same orientation into pUR222. Although hosts carrying this plasmid also lysed when grown in the presence of IPTG, they were sensitive to colicin E2 and produced increased amounts of the 5,000- and 3,000-molecular-weight proteins (but not the full-length immunity protein) when treated with IPTG. The results were consistent with the idea that expression of celB (production of the 5,000- and 3,000-molecular-weight proteins) is sufficient to cause host cell lysis in the absence of colicin production and derepression of the host cell SOS system.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-227839, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-361718, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-378936, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6213604, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6216243, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6269464, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6272193, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6276683, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6293928, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6308394, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6337118, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6403939, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6409986, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6415223, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6444517, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-6455292, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-7035830, http://linkedlifedata.com/resource/pubmed/commentcorrection/6352670-7049397
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
156
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't