Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-7-8
|
pubmed:abstractText |
A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-destaining kinetics for ethidium bromide, linearity of the fluorescence response, and the effect of the molecular topology of DNA on ethidium bromide binding to DNA in agarose.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0147-619X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
9
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
182-90
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:6344110-DNA, Bacterial,
pubmed-meshheading:6344110-Densitometry,
pubmed-meshheading:6344110-Electrophoresis, Agar Gel,
pubmed-meshheading:6344110-Ethidium,
pubmed-meshheading:6344110-Fluorescence,
pubmed-meshheading:6344110-Gene Amplification,
pubmed-meshheading:6344110-Genetic Techniques,
pubmed-meshheading:6344110-Nucleic Acid Conformation,
pubmed-meshheading:6344110-Plasmids,
pubmed-meshheading:6344110-Staphylococcus aureus
|
pubmed:year |
1983
|
pubmed:articleTitle |
Determination of plasmid copy number by fluorescence densitometry.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|