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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-6-10
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pubmed:abstractText |
Acinetobacter calcoaceticus wild-type strain N.C.I.B. 8250 can grow on only the L(+)-isomer of mandelate but mutant strains have been isolated that can grow on D(-)-mandelate. These mutants contain a novel D(-)-mandelate dehydrogenase in addition to the original L(+)-mandelate dehydrogenase. A second wild-type strain, EBF 65/65, shows the opposite pattern and can grow on D(-)-mandelate but not on L(+)-mandelate; mutants have been isolated that possess an L(+)-mandelate dehydrogenase in addition to the original D(-)-mandelate dehydrogenase and can thus grow on L(+)-mandelate. Both L(+)- and D(-)-mandelate dehydrogenases, whether originally present or evolved, are very similar in many respects: they are membrane-bound and NAD(P)+-independent; their activities have similar dependence on temperature and pH; they are inhibited by oxalate but not by several metal-chelating agents; they are stereospecific in their action and are inhibited by the opposite stereoisomers. D(-)-Mandelate dehydrogenase is much more susceptible than L(+)-mandelate dehydrogenase to inhibition by HgCl2 and p-chloromercuribenzoate and is much more heat-labile.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-1001725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-1206374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-13108853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-14852960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-201725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-386722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-4656806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-5660630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-5929747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-6016603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-6029734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-670930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-713837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-778327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-956122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6342613-993779
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
209
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
379-86
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pubmed:dateRevised |
2010-10-13
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Mutant strains of Acinetobacter calcoaceticus possessing additional mandelate dehydrogenases. Identification and preliminary characterization of the enzymes.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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