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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1983-6-10
|
| pubmed:abstractText |
A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, and chromatography on DEAE-cellulose, DEAE-Sephadex, and phosphocellulose. The procedure may be completed in 2.5 days and the resultant enzyme is judged to be greater than 90% pure.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
128
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
294-301
|
| pubmed:dateRevised |
2002-11-1
|
| pubmed:meshHeading |
pubmed-meshheading:6342468-Chromatography, DEAE-Cellulose,
pubmed-meshheading:6342468-Chromatography, Ion Exchange,
pubmed-meshheading:6342468-DNA-Directed RNA Polymerases,
pubmed-meshheading:6342468-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6342468-RNA Polymerase II,
pubmed-meshheading:6342468-Saccharomyces cerevisiae
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Purification of DNA-dependent RNA polymerase II from Saccharomyces cerevisiae.
|
| pubmed:publicationType |
Journal Article
|