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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
|
pubmed:dateCreated |
1983-5-5
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pubmed:abstractText |
Human renal renin was isolated from a partially purified preparation, Haas' preparation step 5 material, with a remarkably high yield of 28% by a newly developed method. This method consisted of only four steps including affinity chromatography on pepstatin-aminohexyl-agarose, chromatofocusing, gel filtration and DEAE-chromatography after the initial extraction and concentration. This method enabled us to obtain pure human renin without another affinity column used by other investigators. Pure human renin had a molecular weight of 40,000 daltons as estimated by gel filtration and sodiumdodecylsulfate-polyacrylamide gel electrophoresis. The pI of purified renin was 5.6.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0024-3205
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
4
|
pubmed:volume |
32
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1591-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6339858-Chromatography, Affinity,
pubmed-meshheading:6339858-Chromatography, Gel,
pubmed-meshheading:6339858-Chromatography, Ion Exchange,
pubmed-meshheading:6339858-Electrophoresis, Disc,
pubmed-meshheading:6339858-Humans,
pubmed-meshheading:6339858-Isoelectric Point,
pubmed-meshheading:6339858-Methods,
pubmed-meshheading:6339858-Molecular Weight,
pubmed-meshheading:6339858-Renin
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pubmed:year |
1983
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pubmed:articleTitle |
A novel purification method of human renin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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